![]() ![]() Primary patient MM cells and bone marrow stromal cells (BMSC)Ĭells were washed with PBS and lysed in RIPA buffer (Cell Signaling Technology) with PMSF (Cell Signaling Technology). ![]() Mycoplasma contamination was checked for using the MycoAlert Mycoplasma Detection Kit (Lonza). Cell lines were used within 3 months after thawing. HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) containing 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin. All MM cell lines were cultured in 5% CO 2 at 37☌ in RPMI1640 medium (Thermo Fisher Scientific) containing 10% FBS (Sigma-Aldrich), 2 mmol/L L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin (Invitrogen). The MM.1R cell line was obtained from Dr. The KMM-1, KMS-11, KMS-12 PE, KMS-21 BM, KMS-26, KMS-27, and KMS-34 cell lines were obtained from JCRB (National Institute of Health Sciences). The 元63, MM.1S, NCI-H929, RPMI8226, and U266 human MM cell lines, as well as the HEK293T human embryonic kidney cell line, were purchased from ATCC. ![]()
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![]() ![]() Together, they make their way across the smoldering wreckage of their homeland to Berlin, where Martin’s mother, the beautiful and naive Benita, has fallen into the hands of occupying Red Army soldiers. ![]() ![]() The widow of a resister murdered in the failed July 20, 1944, plot to assassinate Adolf Hitler, Marianne plans to uphold the promise she made to her husband’s brave conspirators: to find and protect their wives, her fellow resistance widows.įirst Marianne rescues six-year-old Martin, the son of her dearest childhood friend, from a Nazi reeducation home. Set at the end of World War II, in a crumbling Bavarian castle that once played host to all of German high society, a powerful and propulsive story of three widows whose lives and fates become intertwined-an affecting, shocking, and ultimately redemptive novel from the author of the New York Times Notable Book The Hazards of Good Breeding.Īmid the ashes of Nazi Germany’s defeat, Marianne von Lingenfels returns to the once-grand castle of her husband’s ancestors, an imposing stone fortress now fallen into ruin following years of war. Three women, haunted by the past and the secrets they hold ![]() ![]() ![]() Calculus shifted mathematical reasoning from geometric to algebraic patterns of thought, and trigonometry’s participation in this new mathematical analysis grew, encouraging such innovations as complex numbers and non-Euclidean geometry. In the late sixteenth century, trigonometry increasingly entered the physical world through the practical disciplines, and its societal reach expanded with the invention of logarithms. ![]() In Europe, China, and America, trigonometry aided and was itself transformed by concurrent mathematical revolutions, as well as the rise of science and technology.įollowing its uses in mid-sixteenth-century Europe as the “foot of the ladder to the stars” and the mathematical helpmate of astronomy, trigonometry became a ubiquitous tool for modeling various phenomena, including animal populations and sound waves. ![]() Glen Van Brummelen tells the story of trigonometry as it evolved from an instrument for understanding the heavens to a practical tool, used in fields such as surveying and navigation. ![]() The Doctrine of Triangles offers an interdisciplinary history of trigonometry that spans four centuries, starting in 1550 and concluding in the 1900s. ![]() ![]() But Caroline’s past is a dark cellar, filled with repressed memories and a lurking horror that others around her can’t understand.Īs past and present demons converge, Caroline and Lila must chase the source of the unrelenting, oppressive power to its core. As these snarling, teeth-bared delusions begin to take shape in the sculptures she makes in a trance-like state, her fiancé is convinced she needs help from a professional. Soon Lila feels haunted from within, terrorized by a delicious evil that shows her how to find her voice-until she’s in danger for using it.Ģ004: Caroline Sawyer sees dogs everywhere that no one else seems to notice. But when young women around her begin dying, wild speculation ensues. Young women who refuse to conform and don’t know when to shut up.Ģ019: Thirteen-year-old Lila Sawyer has secrets she can’t share with anyone. He’s known as The Cur, and he leaves no trace-except for the victims he most viciously slays every fifteen years. In this biting and electrifying novel from bold horror talent Kristi DeMeester, there’s something out there that’s murdering young women-until an overwhelmed mother and her secretive daughter refuse to live without answers any longer. This one will cut you.” - Christopher Golden Named one of Esquire's Best Horror of the Year One of Goodreads Most Popular Horror of 2022 ![]() ![]() ![]() ![]() ![]() شربیانیĪre you freaking kidding me Sara Shepard? May be this series has been dragged on, and I will be honest, i never minded, because i was still enjoying the series. ![]() However, it is soon revealed that the real A is still seeking vengeance. Hanna gets a film offer, and Spencer starts an anti-bullying campaign. With A apparently arrested, the girls attempt to move on with their lives. Toxic (Pretty Little Liars #15), Sara Shepard ![]() ![]() ![]() He’s readying Naglfar, the Ship of the Dead, complete with a host of giants and zombies, to sail against the Asgardian gods and begin the final battle of Ragnarok. His cousin, Annabeth, recruits her boyfriend, Percy Jackson, to give Magnus some pointers, but will his training be enough? Loki is free from his chains. ![]() Now Magnus faces his most dangerous trial yet. ![]() But he has strong and steadfast friends, including Hearthstone the elf, Blitzen the dwarf, and Samirah the Valkyrie, and together they have achieved brave deeds, such as defeating Fenris Wolf and battling giants for Thor’s hammer, Mjolnir. As the son of Frey, the god of summer, fertility, and health, Magnus isn’t naturally inclined to fighting. Magnus Chase, a once-homeless teen, is a resident of the Hotel Valhalla and one of Odin’s chosen warriors. ![]() ![]() ![]() Now that I have a better idea, I will tell you this: I am an ugly woman." Well, there's considerably more to her than that, and much of "The Sentence" is devoted to figuring out what that is.Ĭonveniently, this endeavor is facilitated by Tookie's having spent her prison time reading voraciously, a learning binge begun with books supplied by her "seventh-grade teacher in the reservation school," Jackie, who happens to work in a Minneapolis bookstore that specializes in Native literature, where Tookie gets a job. But there are ghosts aplenty, and one in particular certainly spooks the novel's Ojibwe narrator, Tookie, whose nickname seems a quick take on her character, tough cookie.Īt the outset, Tookie tells us about having been in prison for 10 years for what seems an almost slapstick crime, committed when "for many reasons, I didn't know who I was yet. I guess you could call Louise Erdrich's new novel "The Sentence" a ghost story, though that implies a certain scary spookiness that the book does not possess. ![]() ![]() ![]() Sometimes change is a force more powerful than will.Īdvance praise from Publishers Weekly: "Medical drama, gossipy lesbian romance, and angsty backstory all get equal time in Radclyffe’s fifth PMC Hospital Romance (after Passionate Rivals).ans of small community dynamics and workplace romance without ethical complications will find this hits the spot. Zoey will never accept second place in matters of the heart, even when her rival is a career, and Dec has nothing left to give of herself or her heart. She’s resolved to protect herself from ever being vulnerable to past mistakes by rebuilding her life around her new role as an ER attending, and that life does not include love. ![]() Zoey Cohen spent a lifetime learning to hide disappointment and loss behind a mask of unshakeable calm and control, even when her longtime friend-with-benefits lover moves on for true love.ĭeclan Black returns to Philadelphia Medical Center after an accident costs her the life she’d built and her career. ![]() ![]() None of the books are required-reading in any Polk County public school. The issue in Polk schools began late last year when County Citizens Defending Freedom found that 16 books the group deems objectionable were available in PCPS media centers. 'This is not a censorship committee': Polk County's book review committees get their orders How it began How it all started: These are the 16 books a conservative group wants banned from Polk public schools librariesĪuthors respond: Writers discuss effort to ban their books in Polk County schools ![]() Heid recommended the book, a coming-of-age novel about a high school student living in extreme poverty in New York City coming to terms with his homosexuality, remain in high school libraries, but also be allowed in middle school media centers, as well. ![]() Documents provided by PCPS show it was checked out one time at Ridge Community High School in the previous school year. ![]() The book had been available at Davenport, George Jenkins, Haines City, Kathleen, Lake Region, and Ridge Community High schools, along with Fort Meade Middle-Senior High School. ![]() Adam Silvera's "More Happy Than Not" was approved by a panel and Superintendent Heid to be allowed in middle and high schools libraries/media centers. ![]() ![]() huh But I'm alone on my sofa bed uh-huh My skin's so soft today But you're so far away And I can go off the memories uh-huh You're starting to feel like a memory. your faith when I need you to be there Askin' for your heart if it's real then it's rare Ocean in the sea GIA I see Lunch time in Cheetah with snow bunny divas On 3 5.La Buena Vida Camila Ca bello-Familia5.La Buena Vida Lyricist Eric FredericÉdgar Barrera Cheche Alara Composer Eric FredericÉdgar Barrera Cheche Alara I woke up h. McCarthy Andre Proctor Floyd Bentley Plenty cry This life without you You don't want you don't want you don't want You don't want you don't want to go back to th. 2 16.BACK TO THE BASICS tor Floyd Bentley Composer Nayvadius Wilburn Montrell MartinezJorge Miguel Cardoso Harissis Tsakmaklis Feliciano Ponce Ecar Luzian Gregor Tuetsch Oli. ![]() ![]() 2] Wrote too much Songs about love When I still ain't found it Wrote too much Songs for my mom But she don't see my vision Tells me school's the safest If you wan. Gareth.T Teddy Fan I shouldn't be as sad as I am But it's out my hands Tryna change up who I am But I don't know if I can but I don't know if I can They say you go. Album ( Page Link ) Song ( Page Link ) ( Partial Lyrics ) 1 1.speed limit reth.T-to be honest1.speed limit Lyricist Gareth.TJacob Strom Composer Gareth.TJacob Strom Arranger Gareth.T Teddy Fan I. ![]() |