![]() ![]() Primary patient MM cells and bone marrow stromal cells (BMSC)Ĭells were washed with PBS and lysed in RIPA buffer (Cell Signaling Technology) with PMSF (Cell Signaling Technology). ![]() Mycoplasma contamination was checked for using the MycoAlert Mycoplasma Detection Kit (Lonza). Cell lines were used within 3 months after thawing. HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) containing 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin. All MM cell lines were cultured in 5% CO 2 at 37☌ in RPMI1640 medium (Thermo Fisher Scientific) containing 10% FBS (Sigma-Aldrich), 2 mmol/L L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin (Invitrogen). The MM.1R cell line was obtained from Dr. The KMM-1, KMS-11, KMS-12 PE, KMS-21 BM, KMS-26, KMS-27, and KMS-34 cell lines were obtained from JCRB (National Institute of Health Sciences). The 元63, MM.1S, NCI-H929, RPMI8226, and U266 human MM cell lines, as well as the HEK293T human embryonic kidney cell line, were purchased from ATCC. ![]()
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